Fakultät für Biologie

Links und Funktionen



Tub-tag labeling: a new approach for site-specific protein labeling

Schumacher et al., 2015; Angewandte Chemie (Leonhardt lab)


A novel chemoenzymatic approach for simple and fast site-specific protein labeling is reported in this publication. Recombinant tubulin tyrosine ligase (TTL) was repurposed to attach various unnatural tyrosine derivatives as small bioorthogonal handles to proteins containing a short tubulin-derived recognition sequence (Tub-tag). This novel strategy enables a broad range of high-yielding and fast chemoselective C-terminal protein modifications on isolated proteins or in cell lysates for applications in biochemistry, cell biology, and beyond, as demonstrated by the site-specific labeling of nanobodies, GFP, and ubiquitin.

(from Abstract)

Versatile and efficient site-specific protein functionalization by Tubulin Tyrosine Ligase.

Dominik Schumacher, Jonas Helma, Florian A. Mann, Garwin Pichler, Francesco Natale, Eberhard Krause, M.Cristina Cardoso, Christian P.R. Hackenberger, Heinrich Leonhardt

(2015) Angewandte Chemie. online 25 Sept. DOI: 10.1002/anie.201505456

Link zur LMU Pressemitteilung